Description
Detection principle
Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.
Performance characteristics
| Synonyms | GSH |
| Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
| Sensitivity | 2 μmol/L |
| Detection range | 2-100 μmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 25 min |
| Precision | Average inter-assay CV: 3.2%Average intra-assay CV: 1.9% |
| Other instruments required | Micropipettor, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
