The 1 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. This ladder consists of 9 individual DNA fragments: 1000, 2000, 3000, 4000, 5000, 6000, 8000, 10000 bp, derived from a mixture of PCR products and specifically digested plasmid DNA. The 5000bp fragment is present at increased intensity for easy identification (concentration of 150ng/5μL); all other fragments appear with equal intensity on the gel (concentration of 50ng/5μL).
|Storage Temperature||4°C for 6 months; -20°C for long time|
- 1. Ready-to-use: premixed with loading dye for direct loading
- 2. Quick reference: 5000 bp (brighter than the other fragments)
- 3. Loading Volume: 2-5 μL / lane
- 4. Please change the electrophoresis buffer in time, and use freshly prepared agarose gel for electrophoresis to ensure good separation results
Guide for Molecular Weight
Take 5 μl DNA ladder in 0.8% agarose gel, use a voltage of 8 V/cm, 1 x TAE Buffer for 40 minutes, and the results are as follows: