Description
Detection principle
Chloride ion in biological fluids are replaced by the mercury ions in mercury thiocyanate through ion replacement, which resulted in the formation of difficult-to-dissociate mercury chloride. The substituted thiocyanate ions were combined with ferric nitrate to form a red complex. The content of chlorine ion can be calculated indirectly by measuring the OD value at 460 nm.
Performance characteristics
| Synonyms | Cl |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 1 mmol/L |
| Detection range | 1.0-60 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 15 min |
| Precision | Average inter-assay CV: 6.4%Average intra-assay CV: 3.6% |
| Other instruments required | Micropipettor, Tubes, Vortex mixer, Incubator, Centrifuge. |
| Storage | 2-8℃ |
| Valid period | 6 months |
