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Alkaline Phosphatase (ALP) Activity Assay Kit

From: 180.00

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Description

Detection principle

Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

Performance characteristics

Synonyms ALP
Sample type Serum,plasma,urine,tissue,cells
Sensitivity 0.2 King unit/100 mL
Detection range 0.2-55.6 King unit/1
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 60 min
Precision Average inter-assay CV: 5.6%Average intra-assay CV: 2.1%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K091-S-100, E-BC-K091-S-500