Description
Detection principle
Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Performance characteristics
| Sample type | Animal liver,muscle |
| Sensitivity | 1.80 mg/g liver tiss |
| Detection range | 1.80-180 mg/g liver |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50 min |
| Precision | Average inter-assay CV: 7.3%Average intra-assay CV: 3.7% |
| Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), Concentrated sulfuric acid |
| Storage | 2-8℃ |
| Valid period | 6 months |
