Description
Detection principle
Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.
Performance characteristics
| Sample type | Animal liver and muscle tissue |
| Sensitivity | 0.06 μg/mL |
| Detection range | 0.06-4.0 μg/mL |
| Detection method | Fluorescence method |
| Assay type | Quantitative |
| Assay time | 50min |
| Precision | Average inter-assay CV: 6.6%Average intra-assay CV: 3.4% |
| Other instruments required | Micropipettor, Incubator, Centrifuge |
| Storage | -20℃ |
| Valid period | 6 months |
