Description
Detection principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms | MDA |
| Sample type | Serum,plasma,animal tissue |
| Sensitivity | 0.38 nmol/mL |
| Detection range | 0.38-133.33 nmol/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 90 min |
| Precision | Average inter-assay CV: 8%Average intra-assay CV: 4.9% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 6 months |
