Description
Detection principle
Reduced glutathione (GSH) can react with dithionitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound which has the maximum absorption peak at 420 nm. The GSH content can be calculated by measuring the OD value at 420 nm.
Performance characteristics
| Synonyms | GSH |
| Sample type | Serum,plasma,tissue,cells |
| Sensitivity | 0.26 mg GSH/L |
| Detection range | 0.26-122.8 mgGSH/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50 min |
| Precision | Average inter-assay CV: 2.4%Average intra-assay CV: 1.8% |
| Other instruments required | Micropipettor, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
