Description
Detection principle
The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Performance characteristics
| Synonyms | T-SOD |
| Sample type | Serum,plasma,hydrothorax,ascites,urine,cells,tissue |
| Sensitivity | 0.2 U/mL |
| Detection range | 0.2 -14.4 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 3.7%Average intra-assay CV: 2.9% |
| Other instruments required | Micropipettor, Multichannel pipettor, Vortex mixer, Incubator |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | Reagent 3: -20℃, others: 2-8℃ |
| Valid period | 12 months |
