Description
Detection principle
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.
Performance characteristics
| Synonyms | TG |
| Sample type | serum, plasma, cells and tissue |
| Sensitivity | 0.14 mmol/L |
| Detection range | 0.14-10 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 9.2%Average intra-assay CV: 4.1% |
| Other instruments required | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) |
| Storage | 2-8℃ |
| Valid period | 6 months |
