Description
Overview
| Product name | APTX Rabbit pAb |
|---|---|
| Catalog No. | A5364 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1). |
|---|---|
| Sequence | LYPYIVEFEEEAKNPGLETHRKRKRSGNSDSIERDAAQEAEAGTGLEPGSNSGQCSVPLKKGKDAPIKKESLGHWSQGLKISMQDPKMQVYKDEQVVVIKDKYPKARYHWLVLPWTSISSLKAVAREHLELLKHMHTVGEKVIVDFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYFLESQAVIEMVQEAGRVTVRDGMPELLKLPLRCHECQQLLPSIPQLKEHLRKHWTQ |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT |
| Calculated MW | 41kDa |
| Observed MW | 40kDa |
Applications
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | 22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji |
| Cellular location | Cytoplasm, Nucleus, nucleolus, nucleoplasm |
Research Area
APTX Rabbit pAb images
Western blot analysis of extracts of various cell lines, using APTX antibody (A5364) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using APTX antibody (A5364).
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (A5364). Western blot was performed from the immunoprecipitate using APTX antibody (A5364) at a dilution of 1:500.
* For research use only. Not for therapeutic or diagnostic purposes.
