Description
Overview
Product name | [KO Validated] CDKN1A/p21CIP1 Rabbit mAb |
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Catalog No. | A19094 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
CloneNo. | ARC51040 |
Background
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Immunogen information
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1). |
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Sequence | MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP |
Gene ID | |
Swiss Prot | |
Synonyms | P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1 |
Calculated MW | 18kDa |
Observed MW | 21KDa/18KDa |
Applications
Reactivity | Human, Mouse, Rat |
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Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
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Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Key application | Western blotting Immunohistochemistry |
Positive samples | MCF7, HeLa, 293T, Mouse large intestine, Mouse testis, Rat lung |
Cellular location | Cytoplasm, Nucleus |
Customer validation |
WB(Homo sapiens, Rattus norvegicus) WB,IHC(Homo sapiens) IHC(Homo sapiens) IF(Homo sapiens, Rattus norvegicus) |
Research Area
- Research Area & PathwaysEpigenetics & Nuclear SignalingDNA Damage & Repair
- Research Area & PathwaysCancerTumor suppressorsp53 pathway
- Research Area & PathwaysSignal TransductionATM Signaling Pathway
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell cycle inhibitors
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell Cycle Control-G1/S Checkpoint
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell Cycle Control-G2/M DNA Damage Checkpoint
- Research Area & PathwaysEndocrine & MetabolismAMPK Signaling Pathway
[KO Validated] CDKN1A/p21CIP1 Rabbit mAb images
Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A19094) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A19094) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
* For research use only. Not for therapeutic or diagnostic purposes.