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GAPDH Mouse mAb

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SKU: GAPDH Mouse mAb - AC002 - ABclonal Category:

Description

Overview

Product name GAPDH Mouse mAb
Catalog No. AC002
Host species Mouse
Purification method Affinity purification
Isotype IgG
CloneNo. AMC0062R

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).
Sequence MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
Synonyms G3PD; GAPD; HEL-S-162eP
Calculated MW 36kDa
Observed MW 36KDa

Reactivity Human, Mouse, Rat
Tested applications WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 – 1:10000
  • IHC-P 1:50 – 1:200
  • IF/ICC 1:50 – 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293T, HepG2, Mouse liver, Mouse spleen, Rat liver, Rat kidney
Cellular location Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Ctenopharyngodon idellus, Danio rerio, Nicotiana tabacum L., Gallus gallus, Fenneropenaeus chinensis, Yeast, Giardia duodenalis, Zika virus, Other, Chlorocebus sabaeus, Capra hircus, Arabidopsis thaliana, fenugreek, Gynura procumbens, Rhinopithecus, Hamster, Boswellia carterii , T. granosa, Carassius auratus, Ovis aries, Chlorocebus aethiops, Mesocricetus auratus, Canis lupus familiaris, Homo s)

IF(Homo sapiens, Mus musculus, Chlorocebus sabaeus, Sus scrofa)

ELISA(Ctenopharyngodon idellus)

IP(Homo sapiens)

WB(Homo sapiens)

IHC(Mus musculus, Homo sapiens)

IF(Mus musculus, Homo sapiens)

Co-IP(Homo sapiens)

Co-IP(Homo sapiens)

IP(Homo sapiens)

IP(Mus musculus)

WB (Homo sapiens)

IHC(Homo sapiens,Rattus norvegicus)

WB，IHC，IF(Homo sapiens)

WB，IF(Mus musculus)

ABclonal:Western blot - GAPDH Mouse mAb (AC002)}

Western blot – GAPDH Mouse mAb (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry – GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded human kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry – GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded mouse kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)}

Immunofluorescence – GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Datasheet

Additional information

CatalogID

AC002-100, AC002-200, AC002-50

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