Description
Overview
| Product name | HEC1/NDC80 Rabbit pAb |
|---|---|
| Catalog No. | A5411 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human HEC1/HEC1/NDC80 (NP_006092.1). |
|---|---|
| Sequence | ETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| Calculated MW | 74kDa |
| Observed MW | 80kDa |
Applications
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | K-562, Lovo, MCF7, 22Rv1, THP-1, 293T, Mouse testis |
| Cellular location | Chromosome, Nucleus, centromere, kinetochore |
Research Area
HEC1/NDC80 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using HEC1/HEC1/NDC80 antibody (A5411) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.
* For research use only. Not for therapeutic or diagnostic purposes.
