Description
Overview
| Product name | IRAK3 Rabbit pAb |
|---|---|
| Catalog No. | A5467 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 367-596 of human IRAK3 (NP_009130.2). |
|---|---|
| Sequence | VLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFCLAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLLPSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVNIDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ASRT5; IRAKM |
| Calculated MW | 68kDa |
| Observed MW | 68kDa |
Applications
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | THP-1, NCI-H125, Rat liver |
| Cellular location | cytoplasm, nucleus, plasma membrane |
Research Area
- Research Area & PathwaysSignal TransductionKinaseSerine/threonine kinases
- Research Area & PathwaysImmunology & InflammationCytokinesInterleukins
- Research Area & PathwaysImmunology & InflammationToll-like Receptor Signaling Pathway
- Research Area & PathwaysImmunology & InflammationCell Intrinsic Innate Immunity Signaling PathwayTLR Signaling
IRAK3 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using IRAK3 antibody (A5467) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.
* For research use only. Not for therapeutic or diagnostic purposes.
