Description
Detection principle
Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Performance characteristics
| Synonyms | Fe |
| Sample type | Serum,tissue |
| Sensitivity | 0.08 mg/L |
| Detection range | 0.29-10 mg/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 25 min |
| Precision | Average inter-assay CV: 2.8%Average intra-assay CV: 1.2% |
| Other instruments required | Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
