Description
Overview
Product name | [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb |
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Catalog No. | A19062 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
CloneNo. | ARC53508 |
Background
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Immunogen information
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human Heme Oxygenase 1 (HO-1/HMOX1) (NP_002124.1). |
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Sequence | MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQA |
Gene ID | |
Swiss Prot | |
Synonyms | HO-1; HSP32; HMOX1D; bK286B10 |
Calculated MW | 33kDa |
Observed MW | 33kDa |
Applications
Reactivity | Human, Mouse, Rat |
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Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
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Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Key application | Western blotting Immunohistochemistry Immunoprecipitation |
Positive samples | HeLa, HepG2, Raw264.7Mouse spleen, Mouse kidney, Rat kidney |
Cellular location | Cytoplasmic side, Endoplasmic reticulum membrane, Microsome, Peripheral membrane protein |
Customer validation |
WB(Rattus norvegicus, Homo sapiens, Mus musculus, Homo sapiens,Mus musculus) |
Research Area
[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb images
Western blot analysis of extracts of Raw264.7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.
Western blot analysis of extracts of HepG2 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.
Western blot analysis of extracts from wild type(WT) and Heme Oxygenase 1 (HO-1/HMOX1) knockout (KO) HeLa cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung using [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit mAb (A19062) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062). Western blot was performed from the immunoprecipitate using Heme Oxygenase 1 (HO-1/HMOX1) antibody (A19062) at a dilution of 1:1000.
* For research use only. Not for therapeutic or diagnostic purposes.