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[KO Validated] Lamin A/C Rabbit mAb

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SKU: [KO Validated] Lamin A/C Rabbit mAb - A19524 - ABclonal Category:

Description

Overview

Product name [KO Validated] Lamin A/C Rabbit mAb
Catalog No. A19524
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5001-08

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1).
Sequence SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS
Gene ID
Swiss Prot
Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Calculated MW 74kDa
Observed MW 68KDa/72KDa

Reactivity Human, Mouse, Rat
Tested applications WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 – 1:600000
  • IHC-P 1:50 – 1:400
  • IF/ICC 1:50 – 1:200
  • IP 1:10000 – 1:120000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thiomersal, 0.05% BSA, 50% glycerol, pH7.3.
Key application Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples HeLa, A-549, NIH/3T3, PC-12
Cellular location Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation

(Caenorhabditis Elegans)

WB(Mus musculus)

IF(Homo sapiens)

WB(Homo sapiens)

Western Blot(Mus musculus)

ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Western blot – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:50000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Western blot – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Western blot analysis of extracts of various cell lines, using [KO Validated] Lamin A/C Rabbit mAb antibody (A19524) at 1:500000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunohistochemistry – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody (A19524) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunohistochemistry – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A19524) at dilution of 1:400 – 1:3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunofluorescence – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunofluorescence – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of L929 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunofluorescence – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunofluorescence – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunofluorescence analysis of U2OS cells using Lamin A/C antibody (A19524) at dilution of 1:100 – 1:1600. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Lamin A/C Rabbit mAb (A19524)}

Immunoprecipitation – [KO Validated] Lamin A/C Rabbit mAb (A19524)

Immunoprecipitation analysis of 300ug extracts of PC-12 cells using 3ug Lamin A/C antibody (A19524). Western blot was performed from the immunoprecipitate using Lamin A/C antibody (A19524) at a dilution of1:100000.

* For research use only. Not for therapeutic or diagnostic purposes.

Datasheet

Additional information

CatalogID

A19524-100, A19524-200, A19524-50

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