Description
Overview
| Product name | [KO Validated] p53 Rabbit mAb |
|---|---|
| Catalog No. | A19585 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC56085 |
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3). |
|---|---|
| Sequence | MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | P53; BCC7; LFS1; BMFS5; TRP53 |
| Calculated MW | 44kDa |
| Observed MW | 53KDa |
Applications
| Reactivity | Human, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunoprecipitation |
| Positive samples | 293T(KO) |
| Cellular location | Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body |
| Customer validation |
WB(Homo sapiens, Mus musculus, Rattus norvegicus, Cyprinus carpio) |
Research Area
- Research Area & PathwaysEpigenetics & Nuclear SignalingTranscription Factors
- Research Area & PathwaysEpigenetics & Nuclear SignalingDNA Damage & Repair
- Research Area & PathwaysProtein phosphorylation
- Research Area & PathwaysCancerTumor suppressorsp53 pathway
- Research Area & PathwaysSignal TransductionErbB-HER Signaling Pathway
- Research Area & PathwaysSignal TransductionMAPK-JNK Signaling Pathway
- Research Area & PathwaysSignal TransductionMAPK-P38 Signaling Pathway
- Research Area & PathwaysSignal TransductionATM Signaling Pathway
- Research Area & PathwaysCell Biology & Developmental BiologyApoptosisMitochondrial Control of Apoptosis
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell cycle inhibitors
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell Cycle Control-G1/S Checkpoint
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell Cycle Control-G2/M DNA Damage Checkpoint
- Research Area & PathwaysEndocrine & MetabolismAMPK Signaling Pathway
- Research Area & PathwaysEndocrine & MetabolismWarburg Effect
- Research Area & PathwaysCell Biology & Developmental BiologyFerroptosis
[KO Validated] p53 Rabbit mAb images
![ABclonal:Western blot - [KO Validated] p53 Rabbit mAb (A19585)}](https://abclonal.com/Uploads/Catalog/A19585/A19585_1.jpg?t=1683355001 "ABclonal:Western blot - [KO Validated] p53 Rabbit mAb (A19585)")
Western blot analysis of extracts from wild type(WT) and p53 Rabbit mAb knockout (KO) 293T(KO) cells, using p53 Rabbit mAb (A19585) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.
![ABclonal:Immunoprecipitation - [KO Validated] p53 Rabbit mAb (A19585)}](https://abclonal.com/Uploads/Catalog/A19585/A19585_2.jpg?t=1683355008 "ABclonal:Immunoprecipitation - [KO Validated] p53 Rabbit mAb (A19585)")
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug p53 antibody (A19585). Western blot was performed from the immunoprecipitate using p53 antibody (A19585) at a dilution of 1:1000.
* For research use only. Not for therapeutic or diagnostic purposes.
