Description
Overview
| Product name | MPG Rabbit pAb |
|---|---|
| Catalog No. | A5410 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
Predicted to enable alkylbase DNA N-glycosylase activity. Predicted to be involved in base-excision repair. Located in cytosol and nucleoplasm.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1). |
|---|---|
| Sequence | MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1 |
| Calculated MW | 33kDa |
| Observed MW | 37kDa |
Applications
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | MCF7, K-562, U-937, Mouse liver |
| Cellular location | Cytoplasm, Mitochondrion matrix, Nucleus, mitochondrion nucleoid |
Research Area
MPG Rabbit pAb images
Western blot analysis of extracts of various cell lines, using MPG antibody (A5410) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 40s.
Immunofluorescence analysis of U2OS cells using MPG antibody (A5410). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (A5410). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of A549 cells using MPG antibody (A5410).
* For research use only. Not for therapeutic or diagnostic purposes.
