Description
Detection principle
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Performance characteristics
| Synonyms | NEFA |
| Sample type | Serum,plasma,tissue homogenate,cells,cell supernatant |
| Detection range | 0.01-3.0 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 6 months |
