Description
Overview
| Product name | Phospho-PKM2-Y105 Rabbit pAb |
|---|---|
| Catalog No. | AP0924 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Immunogen information
| Immunogen | A synthetic phosphorylated peptide around Y105 of human PKM (NP_002645.3). |
|---|---|
| Sequence | ILYRP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30 |
| Calculated MW | 58kDa |
| Observed MW | 58kDa |
Applications
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | NIH/3T3 |
| Cellular location | Cytoplasm, Nucleus |
Research Area
- Research Area & PathwaysEpigenetics & Nuclear SignalingRNA Binding
- Research Area & PathwaysProtein phosphorylation
- Research Area & PathwaysCancerTumor biomarkers
- Research Area & PathwaysEndocrine & MetabolismCarbohydrate metabolism
- Research Area & PathwaysEndocrine & MetabolismWarburg Effect
- ProductsRecombinant ProteinsDrug Targets
Phospho-PKM2-Y105 Rabbit pAb images
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKM2-Y105 pAb (AP0924) at 1:1000 dilution or PKM antibody (A13905).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
* For research use only. Not for therapeutic or diagnostic purposes.
