Description
Detection principle
Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.
Performance characteristics
| Synonyms | Pi |
| Sample type | Serum,plasma,tissue |
| Sensitivity | 0.005 mmol/L |
| Detection range | 0.005-2.0 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 1.3%Average intra-assay CV: 1% |
| Other instruments required | Water bath, Centrifuge, Micropipettor, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 6 months |
