Description
Overview
| Product name | PSMD7 Rabbit pAb |
|---|---|
| Catalog No. | A5356 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-324 of human PSMD7 (NP_002802.2). |
|---|---|
| Sequence | MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFLDHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYISVEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRSYLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKIANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEEKKEKK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | P40; S12; Rpn8; MOV34 |
| Calculated MW | 37kDa |
| Observed MW | 37kDa |
Applications
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | MCF7, SW620, A-549, K-562, BT-474, Mouse skeletal muscle, Mouse heart, Mouse testis |
| Cellular location | cytosol, extracellular exosome, extracellular region, nucleoplasm, nucleus |
Research Area
PSMD7 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using PSMD7 antibody (A5356) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse spleen using PSMD7 antibody (A5356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of MCF-7 cells using PSMD7 antibody (A5356). Blue: DAPI for nuclear staining.
* For research use only. Not for therapeutic or diagnostic purposes.
