Description
Detection principle
Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.
Performance characteristics
| Sample type | Serum,plasma,tissue,cells |
| Sensitivity | 0.006 μmol/mL |
| Detection range | 0.006-2.0 μmol/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 1.5%Average intra-assay CV: 1.3% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
