Description
Detection principle
Production of o-nitrophenol and galactose by o-nitrophenol-β-D-galactoside (ONPG) catalyzed by sodium dependent β-D-galactosidase. The amount of o-nitrophenol is directly proportional to the concentration of sodium ion in the sample. The o-nitrophenol is yellow in alkaline environment. The increase of absorbance is determined at 405 nm, and the content of sodium ion is calculated indirectly.
Performance characteristics
Synonyms | Na |
Sample type | Serum |
Detection range | 80-180 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Storage | 2-8℃ |
Valid period | 12 months |