Description
Detection principle
Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Performance characteristics
| Sample type | Plant tissue |
| Sensitivity | 0.32 μmol/mL |
| Detection range | 0.32-70 μmol/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 40 min |
| Precision | Average inter-assay CV: 8.5%Average intra-assay CV: 3.4% |
| Other instruments required | Micropipettor, Vortex mixer, 100℃ Water bath |
| Other reagents required | PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
