Description
Overview
Product name | CDS1 Rabbit pAb |
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Catalog No. | A13758 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
Background
Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13.
Immunogen information
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human CDS1 (NP_001254.2). |
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Sequence | MLELRHRGSCPGPREAVSPPHREGEAAGGDHETESTSDKETDIDDRYGDLDSRTDSDIPEIPPSSDRTPEILKKALSGLS |
Gene ID | |
Swiss Prot | |
Synonyms | CDS 1 |
Calculated MW | 53kDa |
Observed MW | 57kDa |
Applications
Reactivity | Human |
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Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
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Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. |
Key application | Western blotting |
Positive samples | OVCAR3, U-87MG, 293T |
Cellular location | Endoplasmic reticulum membrane, Multi-pass membrane protein |
Research Area
CDS1 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using CDS1 antibody (A13758) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.
* For research use only. Not for therapeutic or diagnostic purposes.