Description
Overview
| Product name | [KO Validated] ATG5 Rabbit mAb |
|---|---|
| Catalog No. | A19677 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0156 |
Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Immunogen information
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0). |
|---|---|
| Sequence | MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNI |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE |
| Calculated MW | 32kDa |
| Observed MW | 55KDa |
Applications
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunoprecipitation |
| Positive samples | HeLa, SH-SY5Y, Mouse brain, Mouse lung, 293T |
| Cellular location | Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane |
| Customer validation |
(293T、 Hela 、A549、HepG2 、 PC-3、HL-60、RPMI-8226、MLOT-4 ) WB(Spodoptera frugiperda, Mus musculus, Homo sapiens, Human, Rattus norvegicus) Western Blot(Mus musculus) |
Research Area
[KO Validated] ATG5 Rabbit mAb images
![ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)}](https://abclonal.com/Uploads/Catalog/A19677/A19677_1.jpg?t=1679564163 "ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)")
Western blot analysis of extracts from wild type (WT) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (A19677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
![ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)}](https://abclonal.com/Uploads/Catalog/A19677/A19677_2.jpg?t=1679564174 "ABclonal:Western blot - [KO Validated] ATG5 Rabbit mAb (A19677)")
Western blot analysis of extracts of various cell lines, using ATG5 antibody (A19677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
![ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)}](https://abclonal.com/Uploads/Catalog/A19677/A19677_3.jpg?t=1679564202 "ABclonal:Immunohistochemistry - [KO Validated] ATG5 Rabbit mAb (A19677)")
Immunohistochemistry of paraffin-embedded human placenta using ATG5 antibody (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
![ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)}](https://abclonal.com/Uploads/Catalog/A19677/A19677_4.jpg?t=1679564211 "ABclonal:Immunoprecipitation - [KO Validated] ATG5 Rabbit mAb (A19677)")
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (A19677). Western blot was performed from the immunoprecipitate using ATG5 (A19677) at a dilution of 1:1000.
* For research use only. Not for therapeutic or diagnostic purposes.
