Description
Overview
Product name | [KO Validated] Cyclin D1 Rabbit mAb |
---|---|
Catalog No. | A19038 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
CloneNo. | ARC0300 |
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Immunogen information
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385). |
---|---|
Sequence | ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI |
Gene ID | |
Swiss Prot | |
Synonyms | BCL1; PRAD1; U21B31; D11S287E |
Calculated MW | 34kDa |
Observed MW | 34kDa |
Applications
Reactivity | Human, Mouse, Rat |
---|---|
Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
|
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Key application | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
Positive samples | HepG2, MCF7, Mouse lung, HeLa |
Cellular location | Cytoplasm, Membrane, Nucleus |
Customer validation |
WB(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus) Western Blot(Mus musculus) Co-IP(Homo sapiens) WB(Homo sapiens) IF(Mus musculus, Rattus norvegicus) WB,IHC(Homo sapiens) |
Research Area
- Research Area & PathwaysProtein phosphorylation
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCyclins
- Research Area & PathwaysCell Biology & Developmental BiologyCell CycleCell Cycle Control-G1/S Checkpoint
- Research Area & PathwaysCell Biology & Developmental BiologyHedgehog Signaling Pathway
[KO Validated] Cyclin D1 Rabbit mAb images
Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody (A19038) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.
Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody (A19038) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.
Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb (A19038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody (A19038). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (A19038) at a dilution of 1:1000.
* For research use only. Not for therapeutic or diagnostic purposes.