Description
Overview
| Product name | HUS1 Rabbit pAb |
|---|---|
| Catalog No. | A5407 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1). |
|---|---|
| Sequence | MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | hHUS1 |
| Calculated MW | 32kDa |
| Observed MW | 35kDa |
Applications
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | OVCAR-3, K-562, 293T |
| Cellular location | Cytoplasm, Nucleus |
Research Area
HUS1 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using HUS1 antibody (A5407) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.
Immunofluorescence analysis of U2OS cells using HUS1 antibody (A5407). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using HUS1 antibody (A5407). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HUS1 antibody (A5407). Western blot was performed from the immunoprecipitate using HUS1 antibody (A5407) at a dilution of 1:1000.
* For research use only. Not for therapeutic or diagnostic purposes.
