Description
Detection principle
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
Performance characteristics
| Synonyms | HYP |
| Sample type | serum, animal tissue, urine |
| Sensitivity | 0.04 μg/mL |
| Detection range | 0.04-10 μg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50 min |
| Precision | Average inter-assay CV: 4.4%Average intra-assay CV: 1.2% |
| Other instruments required | Test tube, Glass tube, pH test strips. |
| Other reagents required | 6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol |
| Storage | 2-8℃ |
| Valid period | 3 months |
