Description
Overview
| Product name | PP2A-B56γ/PR61γ/PPP2R5C Rabbit pAb |
|---|---|
| Catalog No. | A5480 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human PP2A-B56γ/PR61γ/PP2A-B56γ/PR61γ/PPP2R5C (NP_848702.1). |
|---|---|
| Sequence | MLTCNKAGSRMVVDAANSNGPFQPVVLLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEF |
| Gene ID | |
| Swiss Prot | |
| Synonyms | B56G; PR61G; B56gamma |
| Calculated MW | 61kDa |
| Observed MW | 68-71kDa |
Applications
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | MCF7, K-562, Mouse testis |
| Cellular location | Chromosome, Nucleus, centromere |
Research Area
PP2A-B56γ/PR61γ/PPP2R5C Rabbit pAb images
Western blot analysis of extracts of various cell lines, using PP2A-B56γ/PR61γ/PP2A-B56γ/PR61γ/PPP2R5C antibody (A5480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.
* For research use only. Not for therapeutic or diagnostic purposes.
