Description
Overview
| Product name | TRPM8 Rabbit pAb |
|---|---|
| Catalog No. | A5333 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
Background
Predicted to enable ligand-gated calcium channel activity. Predicted to be involved in calcium ion transmembrane transport and positive regulation of cold-induced thermogenesis. Predicted to act upstream of or within several processes, including cellular calcium ion homeostasis; response to cold; and thermoception. Located in plasma membrane.
Immunogen information
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 980-1104 of human TRPM8 (NP_076985.4). |
|---|---|
| Sequence | GYTVGTVQENNDQVWKFQRYFLVQEYCSRLNIPFPFIVFAYFYMVVKKCFKCCCKEKNMESSVCCFKNEDNETLAWEGVMKENYLVKINTKANDTSEEMRHRFRQLDTKLNDLKGLLKEIANKIK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | TRPP8; LTRPC6; trp-p8; LTrpC-6 |
| Calculated MW | 128kDa |
| Observed MW | 126kDa |
Applications
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | GES1, BGC-823, MGC803, AGS, MKN-45 |
| Cellular location | Cell membrane, Endoplasmic reticulum membrane, Membrane raft, Multi-pass membrane protein |
Research Area
TRPM8 Rabbit pAb images
Western blot analysis of extracts of various cell lines, using TRPM8 antibody (A5333) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.
* For research use only. Not for therapeutic or diagnostic purposes.
